Apparatus and method for gel casting and electrophoresis in a single enclosure
US4954236A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 28, 1989 |
| Grant date | Sep 4, 1990 |
| Priority date | — |
| Expiry date | Jun 28, 2009 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44704
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Slab gels are cast in a gel enclosure which consists of a flat rectangular plate held against a larger plate by a flexible sealing sheet with a removable edge. The smaller plate is retained within raised edges along three sides of the larger plate and rests on a pair of shoulders along two of the raised edges. The sealing sheet spans all three raised edges and the smaller plate resting between them, thereby sealing three sides of the chamber between the plates, the width of the chamber being established by the shoulders. One edge of the sealing sheet is removable to expose the lower end of the gel slab to a buffer solution to permit the performance of an electrophoretic separation in the enclosure once the gel has been formed. A depression in the raised edge underlying the removable edge of the sealing sheet increases the access of a buffer solution to the lower end of the gel slab during electrophoresis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.