Apparatus for separating mononuclear cells from blood and method of manufacturing and using the same
US4954264A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Feb 2, 1989 |
| Grant date | Sep 4, 1990 |
| Priority date | — |
| Expiry date | Feb 2, 2009 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L3/50215
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A device for separating mononuclear cells from blood includes a collection tube, a first layer of a liquid density gradient material placed in the tube, a second layer of a gel-like substance placed in the tube and situated above the first layer, a porous foam member placed in the tube and situated above and in contact with the second layer of gel-like substance, and a third layer of a Newtonian gel-like substance placed in the tube above the porous foam member. The gel-like substance of the second layer has a lower specific gravity than that of the liquid density gradient material of the first layer so that it floats on top of the first layer. The gel-like substance of the second layer becomes less viscous when heated such that it is absorbed by the porous foam member and forms a hydraulic barrier between the liquid density gradient material of the first layer and a blood sample placed in the collection tube.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.