Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA
US4956288A · kind A · utility
63Cited by
2References
20Claims
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Key dates
| Filing date | Apr 22, 1988 |
| Grant date | Sep 11, 1990 |
| Priority date | — |
| Expiry date | Apr 22, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/85
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved method, employing electroporation, for producing novel recombinant host cells characterized by stably integrated foreign DNA at high copy number. These recombinant host cells are useful in the efficient, large-scale production of recombinant proteins and polypeptides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.