Cysteine-depleted muteins of biologically active proteins
US4959314A · kind A · utility
277Cited by
2References
22Claims
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Key dates
| Filing date | Feb 7, 1985 |
| Grant date | Sep 25, 1990 |
| Priority date | — |
| Expiry date | Feb 7, 2005 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Muteins of biologically active proteins such as IFN-.beta. and IL-2 in which cysteine residues that are not essential to biological activity have been deleted or replaced with other amino acids to eliminate sites for intermolecular crosslinking or incorrect intramolecular disulfide bridge formation. These muteins are made via bacterial expression of mutant genes that encode the muteins that have been synthesized from the genes for the parent proteins by oligonucleotide-directed mutagenesis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.