Process of corneal enhancement
US4959319A · kind A · utility
Inventors
Key dates
| Filing date | Feb 17, 1989 |
| Grant date | Sep 25, 1990 |
| Priority date | — |
| Expiry date | Feb 17, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N5/0621
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for establishing functional human corneal tissue consisting of an enhanced contact-inhibited endothelial monolayer derived from isolated human corneal endothelial cells with other integral corneal layers remaining intact. The process is divided into four integral parts. 1. Isolation of human corneal endothelial cells from donor corneas. 2. Establishment of a human corneal endothelial cell line which involves the establishment of primary cell cultures, proliferation, and continued maintenance and subculturing of these cells in vitro. 3. The utilization of long term storage of isolated human corneal endothelial cells at -80.degree. C. 4. Utilizing these isolated corneal endothelial cells from the above processes to enhance the corneal endothelial monolayer of human donor corneas to be used for penetrating keratoplasty. Two distinct methods of corneal endothelial enhancement may be utilized. In the first method, isolated human corneal endothelial cells are incorporated into the existing donor monolayer and re-establish a contact-inhibited functional monolayer, with a flattened, hexagonal configuration. In this procedure, the donor corneal endothelium remains intact and is su…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.