Signal enhancement in assay for an enzyme
US4962024A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 11, 1988 |
| Grant date | Oct 9, 1990 |
| Priority date | — |
| Expiry date | Aug 11, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for assay for an unknown enzyme suspected to be present in a liquid includes signal amplification by use of a second enzyme and a blocked modulator for the second enzyme. Unknown enzyme in the liquid removes a blocking group from the blocked modulator. The resulting modulator activates or inhibits the second enzyme which catalyzes an indicator reaction in which a substrate is converted to a product. The presence or absence of the unknown enzyme in the liquid is indicated by a signal, such as a color change or a rate of color change, associated with the indicator reaction. The concentration of the enzyme in the sample may be determined by the measurement of the signal. The invention includes a kit of materials useful for performing the method of the invention. The method involves the hydrolyzing of a blocked fluoroketone inhibitor to facilitate the analytical method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.