Reagent system for an alpha-amylase assay containing aromatic substituted glycoside

US4963479A · kind A · utility

Assignee

• Hoechst Celanese Corporation · US

Inventors

• Rodrigo G. Chavez
• Harold David · San Diego, US
• Ernest K. Metzner · Del Mar, US
• Gerald Sigler · Carmel, US
• Emily S. Winn-Deen · Poway, US

Key dates

• Filing date: Sep 4, 1987
• Grant date: Oct 16, 1990
• Expiry date: Sep 4, 2007

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2334/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

An aromatic substituted glycoside is disclosed of the formula ##STR1## wherein the configuration of the substituted --OR on the anomeric carbon is alpha, n is an integer of 0 to 1, and R is a substituted aromatic radical selected from the group ##STR2## where R.sub.1 through R.sub.6 are independently halogen, NO.sub.2, SO.sub.3 H, ##STR3## where R.sub.7 is lower alkyl; and includes its stereoisomers, optical isomers and geometric isomers and mixtures of the foregoing isomers. These substrates are useful as direct substrates for alpha-amylases. A process for the preparation of the substrates and related substances is also described.