Method for producing hepatitis B virus proteins in yeast
US4963483A · kind A · utility
17Cited by
1References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 13, 1987 |
| Grant date | Oct 16, 1990 |
| Priority date | — |
| Expiry date | Oct 13, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2730/10122
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The hepatitis B virus preS2 antigen gene linked in one contiguous reading frame to the hepatitis B virus surface antigen gene has been expressed in Saccharomyces cerevisiae utilizing an optimized plasmid construction. The expressed protein aggregates into a particulate form which displays the major antigenic sites encoded by both domains, thereby highlighting the utility of yeast as a host for the high level expression of the preS2 as well as the S domain. This protein is useful in in vitro diagnostic systems and as a vaccine for the treatment and prevention of hepatitis B virus-induced diseases and/or infections.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.