Recombinant DNA clones containing a broad host range gene from Bradyrhizobium japonicum
US4966847A · kind A · utility
10Cited by
0References
4Claims
0Family size
Inventors
Key dates
| Filing date | Jul 27, 1987 |
| Grant date | Oct 30, 1990 |
| Priority date | — |
| Expiry date | Jul 27, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/878
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Recombinant DNA clones according to the invention contain a host range gene of B. japonicum which is contained within a 3.3 kb HindIII restriction fragment from the B. japonicum chromosome. Mutations to this gene change the ability of the bacteria to nodulate different plants. In one example, a Tn5 insertion to this region caused the strain to lose its ability to nodulate siratro, but nodulation of soybeans was unaffected. This gene may be manipulated to extend or restrict the host range of the strain for agricultural purposes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.