Process for isolation of lysozyme and avidin from egg white
US4966851A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 19, 1987 |
| Grant date | Oct 30, 1990 |
| Priority date | — |
| Expiry date | Nov 19, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/815
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A process for the isolation and separation of lysozyme and avidin from egg white is carried out by: PA0 step (a) contacting egg white with a weakly acidic cation exchange resin whereby lysozyme and avidin are adsorbed on to the resin; separating the resin from the egg white and washing the resin to remove residual egg white therefrom; and contacting the washed resin with a low ionic strength eluting buffer whereby lysozyme is eluted from the resin while avidin remains adsorbed on the resin; and PA0 step (b) repeating the complete procedure defined in step (a) for two or more times; and PA0 step (c) finally contacting the resin containing accumulated adsorbed avidin with a high ionic strength eluting buffer whereby avidin is eluted from the resin. Lysozyme and avidin are both commercial products useful, for example, in pharmaceutical applications.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.