Fluorophores for encapsulation into liposomes
US4970074A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 24, 1989 |
| Grant date | Nov 13, 1990 |
| Priority date | — |
| Expiry date | Jul 24, 2009 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/586
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides novel fluorescein derivative compounds having fluorescence spectrum and quantum yield characteristics similar to those of fluorescein. The compounds are readily synthesized and purified and are readily soluble in water at self-quenching concentrations. Significantly, due to the presence of polar polyhydroxy group substituents and the absence of metal-chelating groups, these fluorescein derivatives are susceptible to minimal leakage across liposome membranes and have fluorescence characteristics minimally sensitive to the presence of metal ions. Compounds of the invention are thus exceptionally suitable for use in the development of highly storage stable liposome preparations to be employed in immunolytic assays involving human body fluid samples.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.