Immobilized enzyme electrodes

US4970145A · kind A · utility

Assignee

• Cambridge Life Sciences PLC · GB

Inventors

• Hugh P. Bennetto · London, GB
• Gerard M. Delaney · London, GB
• Jeremy R. Mason · London, GB
• Christopher F. Thurston · London, GB
• John L. Stirling · London, GB
• David R. DeKeyzer · Old Woking, GB

Key dates

• Filing date: Jan 20, 1988
• Grant date: Nov 13, 1990
• Expiry date: Jan 20, 2008

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/817
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Enzyme electrodes are disclosed which are capable of responding amperometrically to the catalytic activity of the enzyme in the presence of its respective substrate and comprising the enzyme immobilized or adsorbed onto the surface of an electrically conductive support member which consists of or comprises a porous layer of resin-bonded carbon or graphite particles, said particles having intimately mixed therewith, or deposited or adsorbed onto the surface of the individual particles prior to bonding to form said layer, a finely divided platinum group metal, thereby to form a porous, substrate layer onto which said enzyme is adsorbed or immobilized and comprising a substantially heterogeneous layer of resin-bonded carbon or graphite particles, with said platinum group metal dispersed substantially uniformly throughout said layer. The preferred substrate materials are resin bonded platinized carbon paper electrodes comprising platinized carbon powder particles having colloidal platinum adsorbed on the surface of the particles and bonded onto a carbon paper substrate using a synthetic resin, preferably polytetrafluoroethylene, as the binder. The preferred enzyme electrodes are glucos…