Processes for separation of DNA fragments
US4971671A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 2, 1987 |
| Grant date | Nov 20, 1990 |
| Priority date | — |
| Expiry date | Mar 2, 2007 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44773
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A process for enabling the separation of a mixture of DNA fragments comprising (1) providing an electrophoresis devive; (2) adding to the device a solution mixture containing DNA fragments of different lengths; (3) energizing the device, thereby creating a sequence of unidirectional uniform electric field pulses therein, said sequence of field pulses alternating between primary positive voltage pulses and secondary pulses of a negative or a positive polarity with less voltage than the primary pulses; (4) calculating the time duration and the field strength required for the primary and secondary field pulses to enable resolution of the fragments into separate and distinct groups corresponding their lengths; and (5) applying in the device the selected primary and secondary fields with intensities and durations corresponding to the size of fragments to be separated. Another embodiment of the invention comprises a computer program that facilitates a DNA gel electrophoresis process.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.