Method of culturing cells using highly gas saturated media
US4973558A · kind A · utility
78Cited by
16References
8Claims
0Family size
Assignee
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Key dates
| Filing date | Apr 28, 1988 |
| Grant date | Nov 27, 1990 |
| Priority date | — |
| Expiry date | Apr 28, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/818
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of culturing cells by providing a highly gassed media on a continuous basis to cells grown in a hollow fiber cartridge includes entraining the media with gas above the gas solubility level of the media. The media is then permitted to rest in a substantially quiescent state so that non-solubilized gas leaves the media. The media is then pressurized prior to delivery to the hollow fibers sufficiently so that the pressure drop and/or flow disturbance in the cell culturing device does not decrease the gas solubility level of the media below the media's gas saturation point.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.