Patent · US Expired

Class II restriction endonuclease KspI and a process for obtaining it

US4975376A · kind A · utility

2Cited by

5References

6Claims

0Family size

Assignee

Boehringer Mannheim GmbH · DE

Inventors

Bryan J. Bolton · Grafton, GB
Michael Jarsch · Bad Heilbrunn, DE
Gudrun Schmitz · Deggendorf, DE
Christoph Kessler · Munich, DE

Key dates

Filing date	Mar 30, 1989
Grant date	Dec 4, 1990
Priority date	—
Expiry date	Mar 30, 2009

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S435/822
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention provides a restriction endonuclease which recognizes palindromic sequences ##STR1## where C* is methylated, and cleaves these sequences at the position indicated by the arrows. This endonuclease is preferably from a microorganism of the genus Kluyvera. The present invention also provides a process for obtaining this new restriction endonuclease and a method for using the endonuclease.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.