Cloning and expression of yeast STE13 and Dpp2 genes encoding dipeptidyl aminopeptidase A and B

US4977085A · kind A · utility

Assignee

• The Board of Regents of the Univ. of California · US

Inventors

• George Sprague · Eugene, US
• Ira Herskowitz · Berkeley, US
• Jeremy W. Thorner · Berkeley, US
• David Julius · San Francisco, US
• Lindley Blair · Los Angeles, US
• Anthony J. Brake · Berkeley, US

Key dates

• Filing date: Feb 29, 1984
• Grant date: Dec 11, 1990
• Expiry date: Feb 29, 2004

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/48
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method is provided for expressing the proteases dipeptidyl aminopeptidases A and B which are useful for processing precursor proteins. Genes controlling these proteases may be insdrted into appropriate vectors and transformed into cultures. The proteases may either be utilized to process precursor proteins in vivo or may be extracted from cultures and used to process precursor proteins in vitro.