Vector comprising signal peptide-encoding DNA for use in Bacillus and other microorganisms
US4977089A · kind A · utility
8Cited by
8References
24Claims
0Family size
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Key dates
| Filing date | Jan 30, 1987 |
| Grant date | Dec 11, 1990 |
| Priority date | — |
| Expiry date | Jan 30, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A novel promoter, ribosome-binding site-encoding DNA, and a number of signal peptide-encoding DNA sequences are described for use in Bacillus and other host cells. The compounds are useful in constructing expression vectors for use in Bacillus and other host cells and drive expression and secretion of proteins such as nuclease and human growth hormone. In addition, a general method for obtaining cells with increased abilities to express and secrete heterologous gene products is described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.