Synthetic affinity ligand compositions and methods for purification and recovery of organic molecules
US4981961A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Sep 8, 1989 |
| Grant date | Jan 1, 1991 |
| Priority date | — |
| Expiry date | Sep 8, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/83
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A novel class of compounds, methods for the preparation thereof and the use thereof in chromatographic methods for binding various biologically active materials non-covalently are disclosed. The class of compounds comprises the reaction product of a polymeric gel with a pyridine base, such as 4-dimethylaminopyridine (DMAP), and a halogen-substituted pyridine, such as 3,5-dichloro-2,4,6-trifluoropyridine (DCTFP), which reaction product may in turn be optionally reacted with hydroxyl ions or specified low-molecular-weight compounds. These compounds are capable of selectively and efficiently binding proteins and other organic materials of interest non-covalently to a degree comparable or superior to the heretofore preferred natural affinity ligands, such as Protein A gels. The novel compounds find particular utility in purification and recovery of proteins such as serum albumin and immunoglobulins of various classes from crude sources, such as diluted serum samples from various species.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.