High gel strength low electroendosmosis agarose
US4983268A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 3, 1989 |
| Grant date | Jan 8, 1991 |
| Priority date | — |
| Expiry date | Aug 3, 2009 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44752
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Purified agarose suitable for rapid electrophoresis, characterized by a sulfate content of less than 0.2 wt % but greater than zero, a pyruvate content of 0-0.1 wt %, and a nitrogen content of 0-0.02 wt %. Gels prepared from the agarose exhibit a gel strength at 1.0 wt % concentration of at least 1200 g/cm.sup.2, substantial absence of DNA binding in 0.07 M or less tris acetate buffer, and an electroendosmosis (EEO) at 1.0 wt % concentration of 0.05 or less. Agaroses are purified to provide the low EEO material by dissolving agarose or alkali-modified agar in an aqueous medium buffered at a pH of 6.0 to 8.0 and containing no more than 2.0 nM salt as chloride, and precipitating the agarose by contact with a lower alkanol.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.