Method for packing chromatographic beds
US4985143A · kind A · utility
5Cited by
19References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 31, 1990 |
| Grant date | Jan 15, 1991 |
| Priority date | — |
| Expiry date | Jan 31, 2010 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/565
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Column chromatography beds are packed through the application of static force. A slurry of the chromatography bed material and a non-viscous liquid is filled into the column plugged at one end, and allowed to settle. The column is transferred to a centrifuge, and centrifuged for a brief period of time to achieve a predetermined packing level, at a range generally of 100-5,000 gravities. Thereafter, the plug is removed, other fixtures may be secured, and the liquid is allowed to flow out through the bed. This results in an evenly packed bed, with no channeling or preferential flow characteristics.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.