Bacteriophage cloning system for the construction of directional DNA libraries
US4987073A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 14, 1990 |
| Grant date | Jan 22, 1991 |
| Priority date | — |
| Expiry date | Mar 14, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/74
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A bacteriophage directional cloning vector containing a multiple cloning site, a method for preparing cDNAs that can be cloned in a unique orientation and a bifunctional oligodeoxynucleotide linker, which creates a unique restriction site when ligated to the 3' end of cDNA fragments. The simple and efficient cDNA cloning system reduces the amount of RNA and effort required for the preparation of large directionally cloned libraries by providing a unique vector that accepts cDNA fragments in a unique 5'-3' orientation and by providing a unique bifunctional linker that facilitates the preparation of cDNA fragments that can be ligated directionally into the vector.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.