Composition and method for in vitro cell culture
US4987079A · kind A · utility
Assignees
Inventor
Key dates
| Filing date | Aug 4, 1989 |
| Grant date | Jan 22, 1991 |
| Priority date | — |
| Expiry date | Aug 4, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/02
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
An improved method and apparatus is disclosed for adding fluids to, or removing fluids from, a body part or organ of an animal which minimizes animal trauma and permits rapid, easy, repeated fluid or low viscosity gel transfers. The apparatus includes a tubular fluid-conveying element adapted for fixed connection to the animal, along with a flexible fluid conduit connected between the element and a specific internal body part or organ, such as a cow's uterus. In use, a syringe is employed to introduce or remove fluids from the body part, through the tubular element and connected conduit. The invention is especially adapted for introducing and recovering cell lines producing monoclonal antibodies or other biologically active products in large mammals, and fascilitates monitoring of antibody production as well as administration of nutrients to enhance cell line growth. In preferred monoclonal antibody production procedures, the cells are initially educated through in vitro contact with ambient fluid of the selected organ (e.g., allantoic fluid from a pregnant host and fetal calf serum), whereupon the educated cells are inoculated and allowed to incubate in the host. It has been found…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.