Culture media package

US4988302A · kind A · utility

Assignee

• Difco Laboratories Incorporated · US

Inventors

• Jerry Wayne Smith · Ann Arbor, US
• Nadine M. Sullivan · Sun Prairie, US

Key dates

• Filing date: Jun 8, 1989
• Grant date: Jan 29, 1991
• Expiry date: Jun 8, 2009

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12M37/04
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A culture media package comprising a container having a bottom wall and a peripheral side wall which is substantially filled with a culture medium, a removable insert over the top wall and having a portion defining a planar undersurface and a removable protective cover over the container and insert. The container and insert have radially extending flanges which are bonded to one another. The package is made by filling the container with a molten culture medium in a sterile environment, bonding the insert to the container in a sterile environment, inverting the sealed container such that the molten medium contacts the planar surface of the insert permitting any gases and bubbles to rise toward a space adjacent the bottom of the container and maintaining the container with the insert thereon in an inverted position until the medium has solidified. The removable cover may be applied to the sealed package of the container and insert either before the sealed package is inverted or after the medium has solidified. The package is preferably made from continuous molded plastic strips of connected containers and connected inserts which are filled with medium and sealed, severed from one ano…