Method for recovering recombinant proteins
US4988798A · kind A · utility
11Cited by
2References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 22, 1990 |
| Grant date | Jan 29, 1991 |
| Priority date | — |
| Expiry date | Jan 22, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N1/06
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for recovering a recombinant protein from a protein solution containing high molecular weight contaminating proteins by directly adding Group IIA metal salts to the solution in amounts sufficient to selectively precipitate the high molecular weight protein contaminants is disclosed. The high molecular weight precipitates are removed and the solution is further processed to remove low molecular weight contaminating proteins and other non-protein contaminants. The recombinant protein is subsequently recovered and further processed to produce a protein composition suitable for its intended use.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.