Hapten-protein conjugates and methods of use in immunoassays

US4990443A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Erasmus Huber · Garching bei München, DE
• Christian Klein · Iffeldorf, DE
• Gunter Pappert · Tutzing, DE
• Klaus Hallermayer · Feldafing, DE

Key dates

• Filing date: Jan 24, 1989
• Grant date: Feb 5, 1991
• Expiry date: Jan 24, 2009

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/961
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides hapten derivatives of the general formula: ##STR1## wherein Hap is a residue formed from a hapten carrying a keto or aldehyde group by splitting off an oxo group and R.sup.1 and R.sup.2, which can be the same or different, are alkyl radicals containing up to 7 carbon atoms and one of the symbols R.sup.1 and R.sup.2 can also represent a hydrogen atom. The present invention also provides hapten-protein conjugates of the general formula: ##STR2## wherein Hap, R.sup.1 and R.sup.2 have the above-given meanings and E--NH is the residue of a protein bound via an .epsilon.-amino group of a lysine residue.