Promoters for the expression of foreign genes in yeast, plasmids comprising them, and use thereof for the production of polypeptides

US4990446A · kind A · utility

Assignee

• Labofina, S.A. · BE

Inventors

• Jacques Oberto · Steenokkerzeel, BE
• John Davison · Brussels, BE

Key dates

• Filing date: Dec 5, 1985
• Grant date: Feb 5, 1991
• Expiry date: Dec 5, 2005

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/81
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Promoters capable of ensuring expression in yeast of genes coding for heterologous polypeptides are disclosed, which comprise a DNA fragment or mutants or sub-fragments thereof, said promoter comprising a definite nucleotide sequence commencing with an EcoRI site and terminated by another restriction site. Also disclosed are vector plasmids containing the promoters, transformed yeasts comprising these plasmids, and a process for preparing polypeptides and especially a 1,4-.beta.-N-acetylmuramidase.