Patent · US Expired

Method for assaying 1,5-anhydroglucitol and kit therefor

US4994377A · kind A · utility

18Cited by
1References
10Claims
0Family size

Assignee

Inventors

Key dates

Filing dateSep 18, 1987
Grant dateFeb 19, 1991
Priority date
Expiry dateSep 18, 2007

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/255
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

This invention relates to a novel method for assaying 1,5-anhydroglucitol, which is expected to serve as a marker for diabetes, and a kit therefor. More particularly, it relates to: PA0 (1) a method for assaying 1,5-anhydroglucitol, which comprises selectively removing sugars from a specimen containing 1,5-anhydroglucitol; allowing pyranose oxidase or L-sorbose oxidase to act on 1,5-anhydroglucitol contained in the sample thus obtained in the presence of oxygen; and determining 1,5-anhydroglucitol from the amount of the hydrogen peroxide thus formed; and PA0 (2) a reagent kit for assaying 1,5-anhydroglucitol which comprises an agent for removing sugars, a reagent for detecting hydrogen peroxide and an enzyme for oxidizing 1,5-anhydroglucitol.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.