Process and medium for cloning and long-term serial cultivation of human endothelial cells
US4994387A · kind A · utility
7Cited by
2References
7Claims
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Key dates
| Filing date | Jun 17, 1988 |
| Grant date | Feb 19, 1991 |
| Priority date | — |
| Expiry date | Jun 17, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/948
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Vigorous growth of endothelial cells from human umbilical vein endothelial cells in vitro is achieved using a culture medium containing endothelial cell growth factor and heparin and/or dextran sulfate. Cell population doubling times of 13 to 21 hours for 60 to 85 populations doublings are obtained, and cloned human endothelial cell strains are established having similar proliferative capacities.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.