Improved process for the purfication of synthetic oligonucleotides

US4997927A · kind A · utility

Assignee

• Gesellschaft fur Biotechnologische Forschung mbH (GBF) · DE

Inventors

• Helmut Blocker · Braunschweig, DE
• Ronald Frank · Lehre, DE
• Gudrun Heisterberg-Moutsis · Heidelberg, DE
• Gisela Kurth · Wolfenbüttel, DE
• Andreas Meyerhans · Barcelona, ES

Key dates

• Filing date: Aug 27, 1985
• Grant date: Mar 5, 1991
• Expiry date: Aug 27, 2005

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/55
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

A process for the purfication of synthetic oligonucleotides is described which drastically simplifies the hitherto very time consuming purification process. The deprotection solution is adsorbed without being concentrated on a carrier having reversed phase properties and optionally ion exchanger properties, the oligonuleotides without lipophilic protecting group are removed, their protecting group is cleaved off from the adsorbed oligonucleotides, the adsorbed oligonucleotides are washed and eluted from the carrier and then purified in a manner known per se.