Propagation process employing agarose medium
US4999299A · kind A · utility
7Cited by
4References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 3, 1988 |
| Grant date | Mar 12, 1991 |
| Priority date | — |
| Expiry date | Jun 3, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S47/11
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention describes a process for culturing proliferating aggregates of plant cells, which comprises (a) uniformly plating isolated protoplasts, or isolated cells regenerated from protoplasts, in or on an agarose-solidified culture medium, and/or (b) cutting this pretreated and solidified culture medium into segments, transferring said segments to a liquid nutrient solution and continuing culturing in both cases until the cell aggregates have attained the desired size.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.