Rehydratable agarose gels
US4999340A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 4, 1988 |
| Grant date | Mar 12, 1991 |
| Priority date | — |
| Expiry date | Aug 4, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC08J2300/10
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention involves a method for separating biological molecules by subjection of said molecules to a separation system in a gel medium. The method most particularly involves the use of a gel slab suitable for such separations which is readily equilibrated with an appropriate solvent for the chosen separation system. An important aspect of the present invention involves the initial preparation of a gel slab comprising between about 0.5% and about 2.0% agarose and between about 1% and about 3.0% linear water-soluble and substantially nonionic polyacrylamide. A preferred gel slab of the present invention contains between about 1% and 3% agarose and about 3% linear, water-soluble, substantially nonionic polyacrylamide. The gel slabs of the present invention are preferably between about 1 mm and 0.5 mm in thickness. Said aged gel slab is then dried to produce a gel precursor sheet. A preferred drying procedure is to subject the gel slab to a stream of warm air to maintain a preferable gel surface temperature of about 45.degree.-50.degree. C., a 0.5 mm gel slab taking about 6-8 min to dry under these conditions. The dried gel precursor sheet, preferably maintained in a dried …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.