Continuous method of refolding proteins
US4999422A · kind A · utility
6Cited by
5References
10Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Apr 15, 1988 |
| Grant date | Mar 12, 1991 |
| Priority date | — |
| Expiry date | Apr 15, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/1136
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is disclosed for continuously treating a solubilized protein solution containing a protein unfolded to some degree, in a small volume continuous flow reactor, to obtain the protein in a conformation exhibiting the protein's characteristic biological activity by continuously diluting out the solubilizing agent, while continuously withdrawing the refolded protein. The continuous process may be carried out using deionized water as a diluent, rather than buffer solutions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.