Method for determination of HIV specific antibodies

US5001049A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Christian Klein · Iffeldorf, DE
• Hubert Bayer · Weilheim, DE

Key dates

• Filing date: Jan 19, 1990
• Grant date: Mar 19, 1991
• Expiry date: Jan 19, 2010

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/974
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a method for determining HIV (human immunodeficiency virus) specific antibodies. A sample is incubated with a solid phase, to which streptavidin is bound in a way to make 0.1-2.5 .mu.g streptavidin per ml of reaction volume available for binding, a biotinylated polypeptide, and a labelled receptor which binds to HIV specific antibodies. The biotinylated polypeptide contains at least one of the amino acid sequences ##STR1## and is from 7 to 50 amino acids long. Following complex formation onto the solid phase, solid and liquid phase are separated, and label is measured in one of these.