PH.phi.29 DNA polymerase
US5001050A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 24, 1989 |
| Grant date | Mar 19, 1991 |
| Priority date | — |
| Expiry date | Mar 24, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/142222
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved method for determining the nucleotide base sequence of a DNA molecule. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA-polymerase which is a .phi.29-type DNA polymerase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.