Human cell line and triomas, antibodies, and transformants derived therefrom
US5001065A · kind A · utility
12Cited by
5References
5Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 27, 1987 |
| Grant date | Mar 19, 1991 |
| Priority date | — |
| Expiry date | May 27, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/948
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A stable, continuous human cell line or progeny thereof is produced that is resistant to 6-thioguanine and ouabain, secretes less than 40 ng/ml of endogenous IgM antibodies, and grows with a doubling time of about 18 hours. The cell line, which preferably is adapted to serum-free medium, may be used as a fusion partner with an antibody-producing cell line so as to generate antibodies. In addition, it may be electroporated with a vector containing a gene of interest to produce a transformed cell line which generates a protein encoded by the gene, such as an IgG or IgM antibody.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.