Production of plasminogen activator from cells to which lectin is added to the culture medium
US5002877A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Feb 22, 1990 |
| Grant date | Mar 26, 1991 |
| Priority date | — |
| Expiry date | Feb 22, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y304/21069
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Increased yields of enzymes, particularly tissue plasminogen activator (tPA), are produced from cells, particularly CNCM I-222 cells, by a process using less concentrated lectin, particularly concanavalin A, in culture medium added to replace initial growth medium than has been used hitherto. After first harvesting of enzyme in supernatants, the culture can be reactivated by the addition of fresh culture medium containing an even lower lectin concentration, and a further harvest of enzyme obtained. The two harvests of enzyme together can give a greater overall yield than a single harvest after a single induction step employing the conventionally used higher lectin concentrations. Enzyme yield can be further increased by repeating the last incubation step once or twice to obtain one or two further harvests.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.