Process for producing human epidermal growth factor and analogs thereof
US5004686A · kind A · utility
15Cited by
2References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 4, 1988 |
| Grant date | Apr 2, 1991 |
| Priority date | — |
| Expiry date | Jan 4, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a process for producing a fusion protein of epidermal growth factor (EGF) attached through a Glu-residue, treatment of the fusion protein with a Staphylococcus aureus protease specific for cleaving peptides at a Glu-linkage thereby producing large amounts of the 53 amino acid EGF sequence. The treatment conditions optimize production of large amounts of the 53 amino acid sequence relative to the 51 amino acid sequence minus the 52 Leu and 53 Arg residues and take full advantage of the selectivity of the protease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.