Purification of hepatitis proteins
US5004688A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 15, 1988 |
| Grant date | Apr 2, 1991 |
| Priority date | — |
| Expiry date | Apr 15, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2730/10122
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention pertains to a process for recovering hepatitis B surface antigen from recombinant Pichia pastoris cells comprising: PA0 (a) lysing said yeast cells in the presence of a buffered chaotropic salt and separating the hepatitis B surface antigen-containing supernatant from said lysed cells; PA0 (b) subjecting the heptatis B surface antigen-containing supernatant obtained in step (a) to conditions suitable to precipitate lipids and contaminant proteins from said supernatant; PA0 (c) subjecting the hepatitis B surface antigen-containing supernatant obtained in step (b) to diafiltration; PA0 (d) contacting the hepatitis B surface antigen-containing retentate obtained in step (c) with silica; PA0 (e) washing contaminant proteins from the resulting silica-adsorbed hepatitis B surface antigen with a buffer having a pH within the range of 6-8; PA0 (f) eluting the hepatitis B surface antigen from the silica with a buffered eluant having a pH within the range of 9.5-11.0 containing from 0.5 to 8 molarity of urea; PA0 (g) subjecting the hepatitis B surface antigen-containing fraction obtained in step (f) to a gel filtration step suitable to separate said hepatitis B surface …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.