Nitroxide labeled nucleotides and nitroxide labeled hybridization probes
US5004809A · kind A · utility
2Cited by
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11Claims
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Key dates
| Filing date | Feb 4, 1986 |
| Grant date | Apr 2, 1991 |
| Priority date | — |
| Expiry date | Feb 4, 2006 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H21/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Nitroxide labeled hybridization probes are formed from novel nucleotides. The novel nucleotides are formed by substituting a nitroxide moiety at the C-5 position of the pyrimidine ring of either cytosine or uracil. The hybridization probes are formed by nick translation with E. coli polymerase I or T4 DNA polymerase or other template dependent enzymes. The hybridization probes can then be detected using electron spin resonance spectroscopy or alternately using a colorimetric method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.