Propagating multiple whole fertile plants from immature leguminous
US5008200A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 23, 1988 |
| Grant date | Apr 16, 1991 |
| Priority date | — |
| Expiry date | May 23, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N5/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Seeds of Glycine max, Glycine soja and hybrids thereof are produced by propagating embryogenic tissue containing multiple immature somatic embryos directly or serially by culturing particular cotyledonary tissue or derivation product thereof on media containing auxin selected from the group consisting of chloro-substituted phenoxyacetic acid, methyl derivative thereof, dicamba or picloram, incubating individual immature somatic embryos or embryogenic tissue containing such in a maturation stage to produce mature somatic embryos, germinating the mature somatic embryos to cause shoot tip formation, cultivating the germinated embryos to provide plantlets with 2 to 5 nodes and roots, cultivating the plantlets to provide whole fertile plants which flower and bear seeds, and recovering the seeds. Culturing to provide immature somatic embryos is carried out utilizing auxin in a concentration within the range of 2.5 to 1000 ppm effective to foster normal development of immature somatic embryos. 2,4-dichlorophenoxyacetic acid is the preferred auxin. Maturation is carried out in B5 or MS medium containing indolebutyric acid and abscissic acid or activated charcoal and sucrose, and germinatio…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.