Lipase immdoilized by cross-linking with inert protein for glyceride synthesis
US5010006A · kind A · utility
2Cited by
5References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 11, 1988 |
| Grant date | Apr 23, 1991 |
| Priority date | — |
| Expiry date | Aug 11, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P7/6454
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A dry porous macromolecular matrix is prepared comprising a cross-linked mixture of 69.9 to 86.6% by weight of an inert protein 0.4 to 10.2% by weight of a lipase enzyme and about 11.7 to 23% by weight of a cross-linking agent. The matrix is prepared by reacting the lipase, inert protein and cross-linking agent in a buffer solution, freezing the resulting reacted mixture at a temperature ranging between -20.degree. C. and -195.degree. C., allowing the frozen mixture to thaw to a temperature ranging from 4.degree. C. to 25.degree. C., and rinsing and drying the resulting product at room temperature.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.