Recombinant DNA-molecules and method for protein production
US5010015A · kind A · utility
Assignees
Inventor
Key dates
| Filing date | Nov 30, 1987 |
| Grant date | Apr 23, 1991 |
| Priority date | — |
| Expiry date | Nov 30, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to recombinant DNA molecules and to methods for producing proteins by means of said molecules. Particularly, the present invention relates to recombinant DNA molecules which are synthetized in Bacillus strain bacteria and are characterized by DNA which codes for exoenzymes excreted by a bacterium of the Bacillus strain and which are present in tens of copies in Bacillus strain bacteria, as well as to recombinant DNA molecules which are modified from the above recombinant DNA molecules and are characterized by DNA containing the regulation and excretion signals of the .alpha.-amylase gene of B. amyloliquefaciens, to which signals a gene of any protein can be joined. These recombinant DNA molecules can be used, for example, to improve the production of .alpha.-amylase in Bacillus strain bacteria, and their modifications to produce any protein in Bacillus strain bacteria.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.