Methods for detecting nucleic acid sequences
US5011769A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 29, 1988 |
| Grant date | Apr 30, 1991 |
| Priority date | — |
| Expiry date | Apr 29, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6876
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides a method for detecting a target nucleic acid which comprises forming a reaction mixture which includes the target nucleic acid and an amount of a complementary single-stranded nucleic acid probe which is greater than the target molecule, under conditions which allow the probe and the target nucleic acid to hybridize to each other and form a double stranded target-probe complex, nicking the hybridized probe at least once within a predetermined sequence so as to form at least two probe fragments hybridized to the target nucleic acid, resulting in the probe fragments to become single-stranded and allowing the target nucleic acid to become hybridized to another probe; and identifying probe fragments, thereby detecting the target nucleic acid. This invention also provides a method for detecting a target nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.