Multiepitopic immunometric assay
US5011771A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 24, 1987 |
| Grant date | Apr 30, 1991 |
| Priority date | — |
| Expiry date | Sep 24, 2007 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/819
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to an immunometric assay for a multivalent antigen in a sample which comprises forming a complex of the antigen together with multiple immobilized monoclonal antibodies against different epitopes of the antigen and with a detectably labeled soluble monoclonal antibody which is identical to one of the multiple immobilized antibodies. The labeled antibody associated with the complex is separated from the remaining soluble antibody and the detectably labeled antibody associated with the complex or unassociated with the complex is detected. Any one of the multiple immobilized monoclonal antibodies shows, by itself, substantially less binding towards the antigen in the immunometric assay, when used with itself or another monocolonal antibody in soluble labeled form, than when used with the multiple immobilized antibodies in combination.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.