Composite yeast vectors
US5013652A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 14, 1986 |
| Grant date | May 7, 1991 |
| Priority date | — |
| Expiry date | Oct 14, 2006 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A DNA sequence comprising: PA1 (a) a hybrid first yeast promoter comprising the functional segment of the DNA sequence encoding the upstream activation site of a gene selected from the group consisting of GAL1, GAL7 and GAL10, operably linked to the functional segment of the transcription initiation site of MF-.alpha.-1; PA1 (b) the functional segment of a signal sequence substantially comprising preferred yeast codons; PA1 (c) the functional segment of the DNA sequence comprising a Saccharomyces GAPDH transcription terminator; PA1 (d) the functional segment of the Saccharomyces 2.mu. circle replication origin; and PA1 (e) the functional segment of the yeast LEU2-d selectable marker. The DNA sequence may further comprise, inserted in phase between the signal sequence and transcription terminator, the functional segment of a DNA sequence encoding a heterologous target peptide. DNA expression vectors comprising these sequences, DNA expression systems comprising these vectors and further comprising a source of the peptide encoded by the DNA sequence of the GAL4 structural gene, yeast hosts transformed with said expression vectors or expression systems, methods of producing heterologou…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.