Compounds for the cleavage at a specific position of RNA, oligomers employed for the formation of said compounds, and starting materials for the synthesis of said oligomers
US5013830A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 12, 1990 |
| Grant date | May 7, 1991 |
| Priority date | — |
| Expiry date | Feb 12, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/10
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
There is disclosed a compound having a double chain which is composed of an RNA (+chain) and a complementary DNA (-chain), wherein a portion of the DNA (-chain) has been replaced by an RNA or a derivative thereof, and wherein, when the compound is subjected to the action of an enzyme having a ribonuclease H activity, it is possible to preferentially cleave the RNA (+chain) in a position corresponding to the unsubstituted portion of the DNA (-chain). The compound can thus be used for the preferential cleavage of a phosphodiester bond in a specific position of RNA. Accordingly, the invention provides a useful means for preparing, for instance, a deletion mutant. There is also disclosed a mixed oligomer which comprises an oligomer of RNA or a derivative thereof and a DNA oligomer, wherein the RNA oligomer or a derivative thereof is conjugated to the DNA oligomer via a phosphate diester linkage between the 5'-hydroxyl group and the 3'-hydroxyl group in the ribose or deoxyribose moiety. There is further disclosed a nucleoside derivative of a given general formula for use as a starting material.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.