DNA vectors and their use in recombinant DNA technology
US5015573A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 5, 1988 |
| Grant date | May 14, 1991 |
| Priority date | — |
| Expiry date | Dec 5, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A new class of DNA vectors, each comprising two replication systems; a first origin of replication resulting in a low copy number and stable inheritance of the plasmid, and a second, high copy number, origin of replication at which replication is directly controllable such that, when host cells carrying the vector are propagated under a first set of conditions, replication takes mainly from the low copy number origin, and that when said cells are propagated under a second set of conditions, replication takes place also from the high copy number origin to produce a high yield of gene product. The controllable origin of replication may be under the control of a natural promoter of RNA transcription or a substitute promoter such as the PL promoter or lac promoter.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.