DNA sequence involved in gene expression and protein secretion, expression-secretion vector including the DNA sequence and the method of producing proteins by using the expression-secretion vector
US5015574A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Nov 20, 1986 |
| Grant date | May 14, 1991 |
| Priority date | — |
| Expiry date | Nov 20, 2006 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A expression-secretion vector is constructed by using a DNA sequence comprising a DNA fragment obtained by subjecting a DNA sequence consisting of a region involved in the expression of a gene coding for an extracellular enzyme of a bacterium of the genus Bacillus and a region involved in the secretion of the enzyme thus expressed to a deletion in the region involved in the secretion which can enhance the extracellular production of a protein dependent on the DNA fragment. By transforming a host bacterium with a recombinant DNA molecule formed by inserting a DNA fragment comprising a gene coding for a desired protein into the expression-secretion vector and then culturing the transformed host, the extracellular production of the desired protein in a large amount can be accomplished.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.