Method for producing the Hinc II restriction endonuclease and methylase
US5015581A · kind A · utility
7Cited by
0References
10Claims
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Assignee
Inventors
Key dates
| Filing date | Mar 15, 1989 |
| Grant date | May 14, 1991 |
| Priority date | — |
| Expiry date | Mar 15, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a method for cloning and producing the Hinc II restriction endonuclease by (1) introducing the restriction endonuclease gene from Haemophilus influenzae Rc into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the Hinc II restriction endonuclease, and (3) purifying the Hinc II restriction endonuclease from the fermented host which contains the vector encoding and expressing the Hinc II restriction endonuclease activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.