Method for in vitro reproduction and growth of cells in culture medium
US5017490A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 10, 1989 |
| Grant date | May 21, 1991 |
| Priority date | — |
| Expiry date | Mar 10, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12M23/24
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Method and apparatus for in vitro culture of cells in culture medium to produce a viable cell mass of cell number greater than the initial cell number, wherein a cell mass of particular cell number is cultured in a first oxygen-permeable culture sub-compartment to an increased cell number, and then aseptically transferred directly from the first sub-compartment to a next serial culture sub-compartment providing additional medium and/or culture space for further growth and reproduction to produce a viable cell mass of still higher cell number. Continued transfers are made, if necessary, to successive serial culture sub-compartment until a viable cell mass of desired cell number is attained, particularly one useful for seeding of a production-scale culture system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.